Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2025
Erindi/veggspjald / Talk/poster V37
Höfundar / Authors: Magnea Sól Sigmarsdóttir, Magnús Arnar Rúnarsson, Pétur Orri Heiðarsson og Kristinn Ragnar Óskarsson.
Starfsvettvangur / Affiliations: Háskóli Íslands
Kynnir / Presenter: Magnea Sól Sigmarsdóttir
The pioneer transcription factor FOXA1 is a key regulator of nuclear hormone receptors and plays a critical role in the progression and prognosis of hormone receptor–positive cancers, such as breast cancer. FOXA1 contains intrinsically disordered regions (IDRs), which harbor mutational hotspots of clinical relevance. One such hotspot is the Wing2 subdomain, where mutations are associated with increased cancer risk and resistance to endocrine therapies. In this project, we investigate how the oncogenic Wing2 mutation S250F alters the activity and structural dynamics of FOXA1 using single-molecule spectroscopy in combination with complementary methods. By elucidating how this mutation enables cancer cells to proliferate in the absence of hormones, we aim to advance understanding of endocrine therapy resistance and uncover how pioneer transcription factors reshape the chromatin landscape.