Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2025
Erindi/veggspjald / Talk/poster V30
Höfundar / Authors: Emily M. Herbert (1), Clément Vaillant (2), Eva María Ingvadóttir (2), Clay Abraham (3,4), Silja Hrönn Hlynsdóttir (2), Sean Michael Scully (2)
Starfsvettvangur / Affiliations: 1. Drexel University 2. University of Akureyri 3. Foundation for Applied Molecular Evolution 4. Bio-Mine LLC
Kynnir / Presenter: Eva María Ingvadóttir
A number of strains of polyhydroxyalkanoate (PHA) producing microorganisms were isolated from marine, geothermally heated intertidal pools in Skjálfandi bay (Húsavík, Iceland). 16S rRNA gene sequence analysis of revealed high similarity (99.9%) to Cobetia amphilecti, a halophilic Gulf of Alaska sponge isolate. Furthermore, whole genome sequence average nucleotide identity (ANI) was found to exceed 95%. Based on preliminary screening, C. amphilecti strain HTP039 was selected for PHA production optimization studies. Here we report the effect of glucose and valeric acid concentration on growth and PHA synthesis of strain HTP039 as well as the influence of nitrogen source and salinity. A number of flocculation agents were investigated ahead of multiliter scale-up experiment where glucose and valeric acid supplemented cultures of strain HTP039 were prepared for extraction yielding a flexible, plastic-like film (~ 30% CDW). NMR analysis results were consistent with that of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV).