Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2025
Erindi/veggspjald / Talk/poster E75
Höfundar / Authors: Gabriele Boretti (1,2), Anna Marczak (3), Helena Hamzehpour (3), Stina Simonsson (4), and Ólafur Sigurjónsson (1,2)
Starfsvettvangur / Affiliations: 1. Department of Engineering, Reykjavik University, Reykjavik, Iceland. 2. The Blood Bank, Landspitali University Hospital, Reykjavik, Iceland. 3. Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland. 4. Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden.
Kynnir / Presenter: Gabriele Boretti
Cartilage degeneration is a leading cause of disability and current therapies do not restore articular cartilage. Biomaterials that combine mechanical support with sustained delivery of pro-regenerative cues offer a path toward disease modification. We present an injectable, bioactive hydrogel: a gelatin-based matrix enriched with lyophilized, pathogen-inactivated human platelet lysate (L-PIPL). This off-the-shelf platelet reservoir within a tunable 3D scaffold enables localized, sustained cytokine delivery while supporting cell viability. Physicochemical characterization (FTIR, nanoindentation, rheology, SEM) showed preserved gel integrity, cartilage-relevant viscoelasticity, and an interconnected porous microarchitecture with signatures from both matrix and platelet components. ELISA confirmed multi-day release of growth factors, including TGF-β1. Encapsulated human chondrocytes (C20A4) maintained high viability and increased activity versus L-PIPL–free controls. These results position L-PIPL hydrogels as a promising disease-modifying platform for cartilage repair.