Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2023
Höfundar / Authors: Gabriele Boretti (1) , Emanuele Giordano (2,3), Mariana Ionita (4,5,6), George Mihail Vlasceanu (4,5), Ólafur Eysteinn Sigurjónsson (1,7), Paolo Gargiulo (1,8), Joseph Lovecchio (1,8)
Starfsvettvangur / Affiliations: 1. School of Science and Engineering, Reykjavík University, 102 Reykjavík, Iceland. 2. Laboratory of Cellular and Molecular Engineering “Silvio Cavalcanti”, Department of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna, 47522 Cesena, FC, Italy. 3. Advanced Research Center on Electronic Systems (ARCES), University of Bologna, 40126 Bologna, BO, Italy. 4. Faculty of Medical Engineering, University Politehnica of Bucharest, 060042 Bucharest, Romania. 5. Advanced Polymer Materials Group, University Politehnica of Bucharest, 060042 Bucharest, Romania. 6. eBio-Hub Research Centre, University Politehnica of Bucharest-Campus, 060042 Bucharest, Romania. 7. The Blood Bank, Landspitali, The National University Hospital of Iceland, 105 Reykjavík, Iceland. 8. Institute of Biomedical and Neural Engineering, Reykjavik University, 102 Reykjavík, Iceland.
Kynnir / Presenter: Gabriele Boretti
Tissue-engineered bone tissue grafts are a promising alternative to the more conventional use of natural donor bone grafts. However, choosing an appropriate biomaterial/scaffold to sustain cell survival, proliferation, and differentiation in a 3D environment remains one of the most critical issues in this domain. Recently, chitosan/gelatin/genipin (CGG) hybrid scaffolds have been proven as a more suitable environment to induce osteogenic commitment in undifferentiated cells when doped with graphene oxide (GO). Some concern is, however, raised towards the use of graphene and graphene-related material in medical applications. The purpose of this work was thus to check if the osteogenic potential of CGG scaffolds without added GO could be increased by improving the medium diffusion in a 3D culture of differentiating cells. To this aim, the level of extracellular matrix (ECM) mineralization was evaluated in human bone-marrow-derived stem cell (hBMSC)-seeded 3D CGG scaffolds upon culture under a perfusion flow in a dedicated custom-made bioreactor system. One week after initiating dynamic culture, histological/histochemical evaluations of CGG scaffolds were carried out to analyze the early osteogenic commitment of the culture. The analyses show the enhanced ECM mineralization of the 3D perfused culture compared to the static counterpart. The results of this investigation reveal a new perspective on more efficient clinical applications of CGG scaffolds without added GO.