Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2023

Biotechnological Potential of Thermophilic Clostridia: Utilization of Caldicellulosiruptor for Chiral Building Blocks

Höfundar / Authors: Sean Michael Scully (1), Eva María Ingvadóttir (1), Jóhann Örlygsson (1),

Starfsvettvangur / Affiliations: 1. University of Akureyri

Kynnir / Presenter: Sean Michael Scully

As 1,2-propanediol (1,2-PD) has a single stereocenter it can be found in two enantiomeric forms. (S)-1,2-PD can be produced microbially from methylpentoses (L-fucose and L-rhamnose) while the (R)-enantiomer is produced from hexoses and pentoses via the methylglyoxal pathway. Few investigations have been reported on thermophiles producing 1,2-PD from deoxysugars although several species within the genus of Caldicellulosiruptor have been reported to do so from L-rhamnose. The aim of this work is to examine the methylpentoses metabolisms of the whole genus of Caldicellulosiruptor. In present investigation all nine strains of Caldicellulosiruptor are examined under anaerobic conditions in batch culture using serum bottles on glucose, fucose and rhamnose (all 20 mM). Rhamnose was used as a carbon and energy source for six of the nine species, with values of 1,2-PD ranging from 0.73 mM (C. owensis) to 7.7 mM (C. hydrothermalis). Other main products were acetate and hydrogen. Fucose was degraded to a mixture of 1,2-PD, acetate, lactate and hydrogen by three (C. bescii, C. saccharolyticus, C. hydrothermalis) of the nine species. C. bescii produced 3.2 mM of 1,2 PD and C. saccharolyticus and C. hydrothermalis 8.0 mM. Our results show that the ability of 1,2-PD-production from methylpentoses is more widespread among the genus Caldicellulosiruptor than previously reported.