Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2021
Erindi/veggspjald / Talk/poster E45
Höfundar / Authors: Thora Bjorg Sigmarsdottir(1), Sarah McGarrity(2), Adrian López García de Lomana (2), Snaevar Sigurðsson(5), James T. Yurkovich (3), Ottar Rolfsson2(), Olafur Eysteinn Sigurjonsson (1, 4))
Starfsvettvangur / Affiliations: (1) School of Science and Engineering, Reykjavík University, Reykjavik, Iceland (2), Center for Systems Biology, University of Iceland, Reykjavik, Iceland (3), Department of Bioengineering, University of California San Diego, La Jolla, CA, USA (4) The Blood Blank, Landspitali – the national University Hospital of Iceland, Reykjavik, Iceland, (5 )Biomedical Center, University of Iceland, Reykjavik, Iceland
Kynnir / Presenter: Þóra Sigmarsdóttir
Mesenchymal stromal cells (MSCs) are multipotent post-natal stem cells with applications in tissue engineering and regenerative medicine. MSCs can differentiate into osteoblasts, chondrocytes, or adipocytes, with functional differences in cells during osteogenesis accompanied by metabolic changes. The temporal dynamics of these metabolic shifts have not yet been fully characterized and are suspected to be of importance for therapeutic applications, such as osteogenesis optimization. Here, our goal was to characterize the metabolic shifts that occur during osteogenesis. We longitudinally profiled five key extracellular metabolites (glucose, lactate, glutamine, glutamate, and ammonia) from MSCs from four donors to classify osteogenic differentiation into three metabolic stages, defined by changes in the uptake and secretion rates of the metabolites in cell culture media. We used a combination of untargeted metabolomic analysis, targeted analysis of 13C-glucose labelled intracellular data, and RNA-sequencing data to reconstruct the gene regulatory network and further characterize cellular metabolism. The metabolic stages identified in this proof-of-concept study provide a framework for more detailed investigations aimed at identifying biomarkers of osteogenic differentiation and small molecule interventions to optimize MSC differentiation for clinical applications.