Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2019
Erindi/veggspjald / Talk/poster V32
Höfundar / Authors: Hermann Dreki Guls (1), Csilla Pelyhe (2), Kristín Ólafsdóttir (3), Halldór Pálmar Halldórsson (1), Sindri Gíslason (4)
Starfsvettvangur / Affiliations: 1. Rannsóknasetur HÍ á Suðurnesjum, Háskóli Íslands, 2. Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary, 3. Rannsóknastofa í lyfja- og eiturefnafræði, Háskóli Íslands, 4. Náttúrustofa Suðvesturlands
Kynnir / Presenter: Hermann Dreki Guls
To estimate feasibility of using N-Atlantic rock crab (Cancer irroratus) as a proxy species for benthic environmental degradation three biomarker responses were investigated in vivo under controlled laboratory exposure of C. irroratus to BaP via ingestion of contaminated prey item, blue mussel (Mytilus edulis), during 21-day experiment. Three biomarker responses as well as accumulated BaP concentrations were measured in hepatopancreas of C. irroratus. Mixed function oxidase (MFO) CYP1A was measured by 7-ethoxy O-deethylase (EROD) activity as an indicator of exposure. Activity of glutathione peroxidase (GPx) and reduced glutathione (GSH) concentrations were implemented as antioxidant response biomarkers to oxidative stress.
Prior to being used as contaminant vector in crab exposure experiment three groups of M. edulis were each exposed to different waterborne BaP concentrations for 14 days at 2, 20 and 50 µg BaP/L respectively. Accumulation of BaP in M. edulis was confirmed by chemical analysis of pooled soft body tissue.
Both, M. edulis and C. irroratus accumulated substantial concentrations of BaP during the experiments. Biological responses in C. irroratus allow for speculation on reliability of implementation of EROD, GPx and GSH as biomarkers for polycyclic aromatic hydrocarbon (PAH) exposure as well as the use of this species in environmental assessment.