Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017
Erindi/veggspjald / Talk/poster V37
Höfundar / Authors: Kristey Bríet Gísladóttir (1,2,3), Sandra Mjöll Jónsdóttir-Buch (1,2,3), Helena Montazeri (1,3), Ólafur Eysteinn Sigurjónsson (1,3,4)
Starfsvettvangur / Affiliations: 1. Platome Líftækni, 2. Háskóli Íslands, 3. Blóðbankinn, 4. Háskólinn í Reykjavík
Kynnir / Presenter: Sandra Mjöll Jónsdóttir-Buch
Human platelet lysates (hPL) are rich in various growth factors, metabolites and chemical mediators that influence and support cellular growth. hPL is used instead of fetal bovine serum to supplement in vitro cell cultures, especially for the growth of mesenchymal stromal cells. hPL can be made from platelet transfusion units by using temperature differences to facilitate platelet rupture and chemical release. Here we evaluated how different temperatures affect the biochemical, metabolic and bioactive profile of hPL over time. hPL was stored from three days up to six months at five different temperatures and evaluated for total protein content, growth factor concentrations, albumin levels, pH and changes in selected metabolites. Their ability to support cell proliferation was confirmed using mesenchymal stromal cells. No differences in total protein concentration, metabolites and pH was observed between temperatures over time. Growth factor concentration for EGF and VEGF was stable over time, but the concentration of bFGF decreased when stored at either 22°C or 37°C for up to 28 days. No difference in cell proliferation was seen when using hPL stored at -20°C or 4°C for up to six months compared to control. hPL stored at 37°C for up to 28 days resulted in poor cell viability. The results indicate that hPL metabolic and biochemical composition remains stable over time at different temperatures with best results obtained when stored at -20°C or 4°C.