Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017
Erindi/veggspjald / Talk/poster V23
Höfundar / Authors: Sigríður Hjartardóttir (1), Þorbjörg Einarsdóttir (1)
Starfsvettvangur / Affiliations: Tilraunastöð Háskóla Íslands í meinafræði að Keldum
Kynnir / Presenter: Sigríður Hjartardóttir
Flavobacteria investigation in Icelandic aquaculture is presented by phylogenic trees built on 16S rRNA and MLST analysis. Samples come from Arctic charr and Atlantic salmon with tail and fin rot symptoms, A. charr fish eggs from hatchery and from intake water on farms.
Flavobacterium psychrophilum is a causative agent in Icelandic aquaculture. F. psychrophylum is found in four A. charr farms and makes a large group of closely related isolates. Isolates from three separate farms give the same MLST results.
Most A. charr fish eggs are from the same hatchery. Our results indicate that F. psychrophilum is not transmitted from hatchery. Many Flavobacteria sp. isolates are however found in fish eggs.
No F. psychrophilum strains were isolated from A. salmon which brach as Flavobacterium sp. with isolates from A. charr of various origin, fish eggs and intake water.
MLST typing gives no evidence of vertical or horisontal transmission of the disease.
Interestingly there are Flavobacteria isolates from two A. charr farms with symptomatic fish that branch separately and are not classified as F. psychrophylum.
We conclude that the main causative bacteria in Arctic charr farming in Iceland is F. pshychrophilum. No evidence is found of vertical or horisontal transmission of Flavobacteria carrying the disease. Many untyped Flavobacteria sp. isolates are found in A. charr and A. salmon. Isolates from A. charr that brance separately on the 16S rRNA tree may indicate a new species.