Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017
Erindi/veggspjald / Talk/poster V19
Höfundar / Authors: Sandra Mjöll Jónsdóttir-Buch (1,2,3), Kathrin Plawecki (4), Christian Christensen (1,2), Ólafur Eysteinn Sigurjónsson (1,3,5)
Starfsvettvangur / Affiliations: 1. Platome Líftækni, 2. Háskóli Íslands, 3. Blóðbankinn, 4. Fachhochschule FH Campus Wien, 5. Háskólinn í Reykjavík
Kynnir / Presenter: Sandra Mjöll Jónsdóttir-Buch
Mesenchymal stromal cells (MSCs) are described to be immunomodulatory and have the ability to suppress peripheral blood mononuclear cells (PBMCs) by cytokine secretion. Here, MSCs were treated with three different types of culture medium supplements: fetal bovine serum (FBS), pathogen inactivated human platelet lysate (PIPL), and freeze-dried pathogen inactivated human platelet lysate (FROST). To analyse the effects on the immunomodulatory capabilities of MSCs, the cells were co-cultured with PBMCs in mixed lymphocyte reaction (MLR) assays.
A significant suppression of PBMC proliferation was observed in PIPL (38.67 ± 3.84 %; p ≤ 0.001, n = 12) and FROST medium (31.9 ± 3.12 %; p ≤ 0.01, n = 12), but not in FBS medium (13.85 ± 11.14 %). However, higher cytokine levels were obtained when FBS medium was used compared to media with human platelet lysate (hPL) supplements, indicating FBS to contain pro-inflammatory molecules, which increase the inflammatory status. There were no significant differences observed between the two hPL media, suggesting FROST to be as suitable as PIPL for large-scale uses.