Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2021
Erindi/veggspjald / Talk/poster E52
Höfundar / Authors: Auður Anna Aradóttir Pind (1,2), Ingileif Jónsdóttir (1,2), Stefanía P. Bjarnarson (1,2)
Starfsvettvangur / Affiliations: 1. Faculty of Medicine, School of Health Sciences, University of Iceland, 2. Department of Immunology, Landspitali - the National University Hospital of Iceland
Kynnir / Presenter: Auður Anna Aradóttir Pind
Immaturity of the immune system contributes to low and transient antibody (Ab) responses in early life partly due to limited survival of bone marrow (BM) plasma cells (PC) and decreased survival factors. Various cells can secrete these factors for PC, which cells are the main producers is still up for debate, especially in early life where this has not been addressed. Therefore we aimed to assess age-dependent maturation of accessory cells of the PC survival niche and their expression of the PC survival factors APRIL and IL-6. Survival niche accessory cells in BM; eosinophils (Eo), macrophages (Mϕ), megakaryocytes (MK), monocytes (Mono), basophils (Baso), lymphocytes (Lym), dendritic cells (DC) and neutrophils (Neu) and their expression of survival factors APRIL and IL-6 in 1, 2 and 3 weeks (3w) old mice were assessed by flow cytometry and compared with adult mice. The frequency and number of Eo, MK, Mono and Neu in BM was lower in 1-3w than adult mice. Lym reached adult levels at 3w and Mϕ reached adult levels at 2w. APRIL expression of BM cells was limited in young mice. In particular, APRIL+ Eo, Mϕ, MK, Mono and Lym had not reached adult levels in 3w old mice. However, IL-6 expression was higher in 1-2w than adult mice where IL-6+ Mϕ, Mono, Neu and Lym were increased. Our study revealed limitations in APRIL expression by accessory cells in neonatal BM which likely contribute to limited survival of PC in early life leading to transient Ab responses in this age group.