Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2021
Erindi/veggspjald / Talk/poster E42
Höfundar / Authors: Sean Michael Scully1, Aaron E. Brown2, Yannick Mueller-Hilger1, Andrew B. Ross2, Jóhann Örlygsson1
Starfsvettvangur / Affiliations: 1. University of Akureyri, Borgir, Norðurslóð, 600 Akureyri, Iceland, 2. School of Chemical and Process Engineering, University of Leeds, Leeds, LS2 9JT, United Kingdom.
Kynnir / Presenter: Jóhann Örlygsson
Thermoanaerobacter species have recently been observed to reduce carboxylic acids to their corresponding alcohols. The present investigation shows that Thermoanaerobacter pseudoethanolicus converts C3-C6 short-chain fatty acids (SCFAs) to their corresponding alcohols in the presence of glucose. The conversion yields varied from 21% of 3-methyl-1-butyrate to 57.9% of 1-pentanoate being converted to their corresponding alcohols. Slightly acidic culture conditions (pH 6.5) was optimum for the reduction. By increasing the initial glucose concentration, an increase in the conversion of SCFAs reduced to their corresponding alcohols was observed. The reduction of SCFAs to their corresponding alcohols was further demonstrated using 13C-labelled fatty acids and the conversion was followed kinetically.