Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017
Erindi/veggspjald / Talk/poster V33
Höfundar / Authors: James T. Yurkovich (1), Daniel C. Zielinski (1), Giuseppe Paglia (2), Ottar Rolfsson (3), Olafur E. Sigurjónsson (4,5), Aarash Bordbar (6) and Bernhard Palsson (1)
Starfsvettvangur / Affiliations: 1. Bioengineering Department, University of California, San Diego,2. Center for Biomedicine, European Academy of Bolzano/Bozen, Bolzano, Italy, 3. Center for Systems Biology, University of Iceland, Reykjavik, Iceland; 4. The Blood Bank, Landspitali-University Hospital, Reykjavik, Iceland 5. School of Science and Engineering, Reykjavik University, Reykjavik, Iceland; and 6. Sinopia Biosciences, San Diego, California.
Kynnir / Presenter: Ólafur E. Sigurjónsson
Introduction
According to standard practice, red blood cells stored for transfusion are kept at 4°C for up to 42 days. Studies on these RBC units therefore require over a month of experimental work. At higher temperatures, dynamics proceed at an increased rate, creating the potential for faster experiments.
Aims
In this study, twelve RBC units were stored in SAGM at 4°C, 13°C, 22°C, and 37°C and profiled using quantitative metabolomics throughout storage in order to determine whether the metabolic RBC response is similar at higher temperatures.
Study design and methods
In order to obtain a rate for each measurement, the profiles were fit as either linear or nonlinear. A quarter of the data (26%) was classified as linear, while approximately half of the measurements (48%) were classified and fit as nonlinear. The remaining 26% of the measurements were either noisy or exhibited no clear trend and were excluded from further calculations. Once a rate was calculated for each measurement, it was plotted on a log2(rate) vs. temperature plot.
Results
Looking at specific pathway behavior, we observed a significant accumulation of metabolites in the nucleotide salvage pathway (e.g., xanthine, hypoxanthine, uridine) at higher temperatures, while concentrations were fairly steady at low temperatures. The same behavior was observed for intracellular citric acid.
Conclusion
Metabolite profiling for RBCs in storage at different temperatures show that the rate of change of metabolite profiles approximately double for every 10°C of change in storage temperature. These results indicate that for metabolic studies, storage at temperatures as high as 13°C provides a similar but accelerated system that could reduce the time required to complete experiments.