Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017

Erindi/veggspjald / Talk/poster E52

Freeze dried platelet lysates from expired platelet concentrates support growth and differentiation of mesenchymal stem cells

Höfundar / Authors: Helena Montazeri (1,2), Sandra Mjöll Jónsdóttir-Buch (1,2,3), Ólafur Eysteinn Sigurjónsson (1,2,3,4)

Starfsvettvangur / Affiliations: 1. Platome Líftækni, 2. Blóðbankinn 3. Háskóli Íslands, 4. Háskólinn í Reykjavík

Kynnir / Presenter: Helena Montazeri

Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow. They have the ability to differentiate into osteogenic, chondrogenic, and adipogenic lineages. MSCs are envisioned for use in clinical regenerative medicine. They are typically expanded in media supplemented with fetal bovine serum (FBS). That is not desirable for clinical applications. Platelet lysates, made from expired platelet transfusion units, can substitute FBS as a media supplement in the expansion of MSCs. Here we evaluate whether platelet lysates can be freeze-dried to improve stability and storage potential without affecting the bioactivity. Human bone marrow MSCs from three donors were expanded in media supplemented with lysates manufactured from expired pathogen-inactivated platelet concentrations (PIPL) or lyophilized version of those lysates (Lyo-PIPL). The morphology, expansion, and tri-lineage differentiation of MSCs were analyzed. The proliferation of MSCs in Lyo-PIPL had denser growth than MSCs in PIPL. The differentiation of MSCs into osteogenic, chondrogenic, and adipogenic lineage was similar between media supplements. Our results indicate that MSCs can differentiate into osteogenic, chondrogenic, and adipogenic lineage with both PIPL and Lyo-PIPL as a media supplement.