Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017

Erindi/veggspjald / Talk/poster E21

Icelandic ovine Mycoplasma ovipneumoniae are variable bacteria that induce limited immune responses in vitro and in vivo

Höfundar / Authors: Þorbjörg Einarsdóttir (1), Sigríður Hjartardóttir (1)

Starfsvettvangur / Affiliations: 1. Tilraunastöð HÍ í meinafræði að Keldum

Kynnir / Presenter: Þorbjörg Einarsdóttir

Mycoplasma ovipneumoniae is a pathogen that causes atypical pneumoniae in sheep and goats. While infection of lambs can induce strong immune responses, typically measured as serum antibodies, experimental vaccines generally induce much lower antibody titers. Improved vaccination strategies rely on better understanding of the bacterium and its interaction with the host. In this study, we designed primers to compare seven M. ovipneumoniae gene sequences, in addition to the 16S sequence typically used, to estimate the variability between isolates. In addition, we labeled bacteria with a two-step process and found that the bacteria could be intracellular as well as on the host cell surface in vitro. This indicates that an optimal vaccine should induce both cellular and humoral responses. However, four vaccinations of sheep produced neither significant humoral or cellular responses in this study, likely due to previous exposure of the animals to mycoplasmas. We conclude that an alternative vaccination strategy, e.g. utilizing a recombinant vaccine, may be needed in endemic regions and we suggest a possible vaccine candidate.