Líffræðifélag Íslands
Líffræðiráðstefnan 2015
Erindi/veggspjald / Talk/poster E40
MIRIAM GUADALUPE CONTRERAS MOSTAZO
1. Haskoli íslands, 2.Stofnfiskur
Kynnir / Presenter: Miriam Guadalupe Contreras Mostazo
Tengiliður / Corresponding author: Miriam Guadalupe Contreras Mostazo (miriam@stofnfiskur.is)
The main aim of this project was to test if increased expression of AMP and iNOS genes due to three different immunostimulants (HMB, PBA and vitaminD3) has a defense role against bacterial and virus infection. Once we had established conditions for inducing the expression of these AMPs and iNOS, we conducted a study in which a calculated number of 24-wells plates containing CHSE-214 cells, ASK cells and RTS-11 cells grown to 80% confluency, were pre-treated for 48h with the inducers HMB, PBA and vitaminD3. After this pre-treatment plates with CHSE214 were infected with infectious pancreatic necrosis virus (IPNV) and ASK cells with infections salmon anemia virus (ISAV) at a multiplicity of infection (MOI) of 0.1 (0.1 plaque forming units per cell) in a reference laboratory in Chile. In addition, a CHSE-214 cell plate and a RTS-11 cell plate were infected with Pisciricketssia salmonis in a different reference laboratory, also located in Chile. The findings of this study strongly suggest that pre-treating cells with concentrations of HMB, known to induce expression of AMPs and iNOS, can lower IPNV and ISAV infectivity by 53% and 26% respectively. PBA and vitaminD3 treatments worked similarly by reducing the number of infected cells with IPNV by 17 and 20 percent respectively. Contrarily, they did not reduce the number of infected cells with ISAV and, interestingly, vitaminD3 increased the number of infected cells rather than decrease it. Unfortunately dilution of bacterial infection used for P. salmonis caused a violent infection and, at the moment of analysis, our internal housekeeping gene control was severely degraded preventing us from drawing any conclusions.