Líffræðifélag Íslands
Líffræðiráðstefnan 2015
Erindi/veggspjald / Talk/poster E34
Óttar Rolfsson, Manuela Magnúsdóttir, Skarphéðinn Halldórsson, Ólafur E. Sigurjónsson and Steinn Guðmundsson.
Háskóli Íslands
Kynnir / Presenter: Óttar Rolfsson
Tengiliður / Corresponding author: Óttar Rolfsson (ottarr@hi.is)
Mass spectrometry based metabolomic analysis techniques have recently come of age and are now rutinely used to support the exploration of various biological events. For the past 4 years we have carried out various mass spectrometry based analytical efforts of diverse human cell types and built metabolic networks that allow the analysis of these data using computational systems biology techniques. These include projects aimed at understanding the fundamentals of red cell metabolism and platelet activation during storage within a blood bank, the metabolism of breast epithelium and endothelial cell metabolism. We have also applied mass spectrometry to determine enzyme activities and explore enzyme substrate specificity. Here I will report these efforts and demonstrate how targeted and non-targeted metabolomic analysis of cell media and cell extracts can be applied to support more traditional molecular biology based methods commonly employed to probe cell biology events.