Líffræðifélag Íslands
Líffræðiráðstefnan 2013
Erindi 31

Metagenomic natural product discovery in lichen provides evidence for a family of biosynthetic pathways in diverse symbioses

Andrey N. Gagunashvili (1), Annette Kampa (2), Tobias A. M. Gulder (2), Brandon I. Morinaka (2), Cristina Daolio (3), Markus Godejohann (3), Vivian P. W. Miao (4), Jörn Piel (2,5) og Ólafur S. Andrésson (1)

1) Faculty of Life and Environmental Sciences, University of Iceland, 101 Reykjavik, Iceland
2) Kekule Institute of Organic Chemistry and Biochemistry, University of Bonn, 53121 Bonn, Germany
3) Bruker BioSpin GmbH, 76287 Rheinstetten, Germany
4) Department of Microbiology and Immunology, University of British Columbia, V6T 1Z3 Vancouver, Canada
5) Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, 8093 Zurich, Switzerland

Kynnir: Andrey N. Gagunashvili
Tengiliður: Ólafur S. Andrésson (osa@hi.is)

Bacteria are a major source of natural products that provide rich opportunities for both chemical and biological investigation. Although the vast majority of known bacterial metabolites derive from free-living organisms, increasing evidence supports the wide-spread existence of chemically prolific bacteria living in symbioses. A strategy based on bioinformatic prediction, symbiont cultivation, isotopic enrichment, and advanced analytics was used to characterize a unique polyketide, nosperin, from a Nostoc sp. cyanobacterium associated with the lichen Peltigera membranacea. The biosynthetic gene cluster and the structure of nosperin, determined from 30 μg of compound, are related to those of the pederin group previously known only from nonphotosynthetic bacteria associated with beetles and marine sponges. The presence of this natural product family in such highly dissimilar associations suggests that some bacterial metabolites may be specific to symbioses with eukaryotes and encourages exploration of other symbioses for drug discovery and better understanding of ecological interactions mediated by complex bacterial metabolites