Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017

Erindi/veggspjald / Talk/poster E54

Development of an innovative bioreactor system for human bone tissue engineering

Höfundar / Authors: Joseph Lovecchio (1,2), Emanuele Giordano (2,3,4), Paolo Gargiulo (1,5), Ólafur Eysteinn Sigurjónsson (1,6)

Starfsvettvangur / Affiliations: 1. Institute of Biomedical and Neural Engineering, Reykjavík University, Iceland. 2. Laboratory of Cellular and Molecular Engineering “Silvio Cavalcanti” - Department of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna, Via Venezia 52, 47521 Cesena (FC), Italy. 3 Health Sciences and Technologies - Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Via Tolara di Sopra 41/E, 40064 Ozzano dell’Emilia (BO), Italy. 4. Advanced Research Center on Electronic Systems (ARCES), University of Bologna, Via Vincenzo Toffano 2/2, 40125 Bologna, Italy. 5. Department of Science, Landspítali University Hospital, Reykjavík, Iceland. 6. The Blood bank, The Landspitali University Hospital, Reykjavík Iceland

Kynnir / Presenter: Joseph Lovecchio

In the past two decades significant progress has been carried out in the field of biomaterials and cell therapy leading to significant advances in the development of engineered tissues. Normally, the right approach consists to take in account three fundamental components: a scaffold useful as substrate for tissue growth and development, a source of cells to facilitate required tissue formation, growth factors and/or biomechanical stimuli to address the differentiation of cells within the scaffolds.
In this project a mechanical actuator system (i.e. a bioreactor) was designed and constructed with the aim of using it to enhance cell survial and and osteogenic differentiation of human mesenchymal stromal cells (MSC), seeded on chitosan/graphene (CHT/G) scaffolds. The Scaffolds were cultured using a) medium perfusion, as a stimulus increasing diffusive metabolic exchanges from/to the cells, and b) mechanical stress (compression), as a stimulus mimicking the natural environment inducing osteogenic tissue differentiation or c) in a static cell culture environment Qualitative and quantitative analysis were performed in order to evaluate: (i) cell distribution and viability inside of the CHT/G scaffolds (Live/Dead Assay); (ii) Extracellular matrix (ECM) mineralization (Von Kossa staining).
Preliminary data confirm that the bioreactor system is apt to transfer to the scaffold a relevant mechanical cue which is translated into a sizable biological effect.