Líffræðifélag Íslands - biologia.is
Líffræðiráðstefnan 2017

Erindi/veggspjald / Talk/poster E53

Analysis of the lipid content of the Kerecis Omega3 xenograft and comparison to other skin grafts

Höfundar / Authors: Hulda S. Jónasdóttir (1), Ingibjörg L.K Hafliðadóttir (1), Skúli Magnússon (1,2), Óttar Rolfsson (1)

Starfsvettvangur / Affiliations: 1. Center for Systems Biology, University of Iceland, 2. Kerecis, Iceland

Kynnir / Presenter: Hulda Soffía Jónasdóttir

Background: Tissue-based products derived from mammals require heavy processing due to disease transmission risk. Such harsh processing methods remove most of the tissue’s natural components, leaving behind only a matrix of the most insoluble collagens.
Clinical studies have shown that fish skin* grafts promote faster healing and provide and a pain relieving effect. Human skin and fish skin* are similar due to evolutionary homology. Cold water fish contain omega-3 polyunsaturated fatty acids (PUFAs), precursors of the specialized pro-resolving lipid mediators (SPM) that have a role in host defense, remodeling of tissue and in the mechanism of pain responses.
Aim: To compare the total fatty acid (FA) content and lipid profile in fish skin grafts*, donor cadaver human skin, and bovine collagen matrix#.
Method: Lipids were extracted using liquid-liquid extraction. Fatty acid analysis was done with gas chromatography and lipid analysis with liquid chromatography-mass spectrometry.
Results: Preliminary results show that omega-3 PUFAs in fish skin are >30% of total FAs but <1% in lipids from human skin and bovine collagen matrix. Total lipid profile of the fish skin is distinctively different from the mammalian products.
Conclusion: Data presented here shows abundant omega-3 fatty acids in the fish skin, which may (via production of SPM or other pathways) contribute to faster healing.
Trademarked Items: * Kerecis™ Omega3 by Kerecis # Veritas® Collagen Matrix by Baxter