Líffræðifélag Íslands
Líffræðiráðstefnan 2015
Erindi/veggspjald / Talk/poster E39
Rosana A. Estévez
Háskóli Íslands
Kynnir / Presenter: Rosana A. Estévez
Tengiliður / Corresponding author: Rosana A. Estévez (rae26@hi.is)
Abstract The innate immune response in fish is of particular relevance for infections as it forms the first line of defense against bacteria and viruses. Antibiotics and vaccination are the most common method used to control the infectious diseases in aquaculture. The disadvantages with these approaches are the selection of resistant bacteria and poor protection. Defining inducers for the innate immunity in fish could provide alternative approach against infections. The expression of innate effectors: cathelicidin, hepcidin, iNOS, MDA5, RIG-I and ISG25 mRNA was assessed by qPCR in Chinook salmon cell line (CHSE-214) before and after treatment with potential inducers: ß-hydroxy-ß-methylbutyrate, 4-phenylbutyrate, 1,25(OH)2 D3, omega-3 and omega-3 ethyl ester from fish oil and cod liver oil. Cathelicidin was found to be up-regulated by all the inducers, while the other innate immune effectors were found up-regulated by specific inducers.